Figure 5.
TEM imaging and EDS elemental graph of AgNPs. Figures (A, C) were the TEM images of the stratum corneum layer and cell layer of 3D EpiKutis® respectively, of which EDS energy peaks of the detected elements were shown in the figure (B, D) correspondingly. Figure (E) was the TEM image of 2D KC cells, of which EDS energy peaks of the detected elements were shown in the figure (G). Figure (F) was the magnified TEM dark-field image of the object in the square frame of figure (E). The 3D EpiKutis and 2D KC cells were exposed to the equivalent doses of 0.28 ng AgNPs per cell. The scale bar in the figure (F) was 200 nm, the scale bar in other figures was 500 nm.