Table 1.
Primers used for sequencing and cloning assays
| Target gene/primers | Sequence (5′–3′) | Size (bp) |
|---|---|---|
| lafB-like gene, E. faecium | ||
| LafB2_F | GTATCCGTGTGCCGATTCAA | 337 |
| LafB2_R | GCACCTGAATACGCACCTT | |
| dak_SNP239, E. faecium | ||
| Dak239_F | GAAATGGTCCAGGCAGGTG | 362 |
| Dak239_2_R | AATCGTTCCTTCTACTGGTTTCA | |
| dak _SNP 777delA, E. faecium | ||
| Dak777_2F | TCATCGCAGTGTCCAAGGTC | 386 |
| Dak777_2R | GGACGCCTTTACCAGCAG | |
| lafB-like gene (cloning and sequencing) | ||
| LafB_F (KpnI) | GGTACCGTGAAGGGGGACGTAAGGTG | 1084 |
| LafB_R (XbaI) | TCTAGACTCCAAACCTAGTCCTTGACCT | |
| dak (cloning and sequencing) | ||
| Dak_F (KpnI) | GGTACCAGCAGTCGATCTGAAGGAGGA | 1814 |
| Dak_R (XbaI) | TCTAGAAAAAGGACTTGGTCAGCGAT | |
| To verify gene in the plasmid vector | ||
| pAT28_F | TAATGCAGCTGGCACGACAGG | 552 |
| pAT28_R | GCGTGATTGCCAAGCACGTCC | |