Table 3.
Strain/Ref contig | Ref position (bp) | Variant type | Ref allele | Mutant allele | Amino acid change | Annotation |
---|---|---|---|---|---|---|
HBSJRP9 (daptomycin MIC 8 mg/L) | ||||||
25 | 866 | insertion | — | CTTGGTCTTCT | frame shift | hypothetical protein |
32 | 5333 | deletion | T | — | NA | intergenic (between sensor histidine kinase VanS and contig break) |
41 | 19814 | SNP | T | A | NA | intergenic (between mobile element protein and contig break) |
75 | 8628 | NSY SNP | C | T | Gly106Glu | PTS system, sucrose-specific |
106 | 26313 | SNP | T | C | NA | intergenic (between S-adenosylmethionine:tRNA ribosyltransferase-isomerase and contig break) |
123 | 30223 | NSY SNP | C | T | Thr112Ile | cell division protein FtsI |
144 | 5948 | NSY SNP | G | A | Arg208His | SSU ribosomal protein S2p |
145 | 9634 | insertion | — | ATCTGTCTCGCAG | NA | intergenic (between methionine ABC transporter ATP-binding protein and CsbD-like protein) |
HBSJRP10 (daptomycin MIC 4 mg/L) | ||||||
59 | 22691 | NSY SNP | A | T | Lys233Ile | multimodular transpeptidase-transglycosylase |
66 | 29793 | deletion | G | — | NA | intergenic (between GCN5-related N-acetyltransferase and PTS system, cellobiose-specific IIC component) |
75 | 13399 | SYN SNP | G | A | NA | mobile element protein |
130 | 23788 | SYN SNP | T | C | NA | ribosomal protein S18 |
HBSJRP12 (daptomycin MIC 8 mg/L) | ||||||
17 | 7425 | SYN SNP | G | A | NA | hypothetical protein |
17 | 19639 | NSY SNP | T | A | Asp518Glu | metal-transporting ATPase |
32 | 5333 | deletion | T | — | NA | intergenic (downstream of sensor histidine kinase VanS, upstream of contig break) |
106 | 26313 | SNP | T | C | NA | intergenic (between S-adenosylmethionine:tRNA ribosyltransferase-isomerase and contig break) |
HBSJRP13 (daptomycin MIC 2 mg/L) | ||||||
106 | 6463 | NSY SNP | C | T | Thr316Met | d-alanine-d-alanine ligase |
NSY SNP, non-synonymous SNP; SYN SNP, synonymous SNP; NA, not applicable (no change in coding sequence; shared between strains).
Bold text indicates that this change is hypothesized to cause the lower daptomycin MIC.