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. 2019 Nov 25;56(1):243–257. doi: 10.3892/ijo.2019.4919

Figure 4.

Figure 4

Effects of LINC00460 expression on EMT and cell migration. (A) Changes in gene expression levels of ZEB1, vimentin (VIM) and E-cadherin (CDH1) were measured in PC9-GR and engineered H1299 cells. The results of RT-qPCR are presented as the means ± SD (n=3). (B) Immunofluorescence was used to observe changes in expression levels of vimentin and E-cadherin in engineered H1299 cells. DAPI was used to stain the cell nuclei. (C) Western blot analysis was used to observe changes in activation or expression levels of EMT-related proteins in the indicated cells. β-actin was used as a control. (D) Western blot analysis of the expression of EMT-related proteins and downstream proteins of IL-6 following transient transfection with LINC00460 and treatment of PC9 cells with rh IL-6 (50 ng/ml) or untreated PC9-GR cells. (E) Wound healing assays of PC9-GR cells transfected with siRNAs targeting LINC00460 (left panel) or engineered H1299 cells (right panel). Data for the healing areas are presented as the means ± SD (n=3). WT, wild type; KO, knockout; EV, empty vector; OE, overexpression; rhIL-6, recombinant human interleukin-6. The level of statistical significance was set at P-value <0.05 (**P<0.01, ***P<0.001). n.s., not significant.