Table II.
List of antibodies used in this study.
| Antibody | Manufacturer | Species | Dilution |
|---|---|---|---|
| p-EGFR (Y1068) | Abcam (ab5644) | Rabbit monoclonal | 1:1,000 for WB |
| EGFR | Abcam (ab52894) | Rabbit monoclonal | 1:1,000 for WB |
| p-STAT3 (Y705) | Abcam (ab76315) | Rabbit monoclonal | 1:2,000 for WB |
| STAT3 | Abcam (ab68153) | Rabbit monoclonal | 1:2,000 for WB |
| p-AKT (S473) | Cell Signaling Technology (#4060) | Rabbit monoclonal | 1:1,000 for WB |
| AKT | Cell Signaling Technology (#9272) | Rabbit monoclonal | 1:1,000 for WB |
| p-ERK1/2 (T202/Y204) | Cell Signaling Technology (#4370) | Rabbit monoclonal | 1:2,000 for WB |
| ERK1/2 | Cell Signaling Technology (#9102) | Rabbit monoclonal | 1:1,000 for WB |
| GAPDH | Abcam (ab9485) | Rabbit monoclonal | 1:1,000 for WB |
| Lamin B1 | Abcam (ab65986) | Rabbit monoclonal | 1:1,000 for WB |
| Normal Rabbit IgG | Sigma-Aldrich (12-370) | Rabbit | 1:50 for RIP |
| FLAG (M2) WB |
Sigma-Aldrich (F1804) | Mouse monoclonal | 1:50 for RIP, 1:1,000 for |
| Vimentin (V9) | Santa Cruz Biotechnology (sc-6260) | Mouse monoclonal | 1:1,000 for WB, 1:100 for IF |
| E-cadherin (G-10) | Santa Cruz Biotechnology (sc-8426) | Mouse monoclonal | 1:1,000 for WB, 1:100 for IF |
| Cleaved Caspase-3 (Asp175) (5A1E) | Cell Signaling Technology (#9664) | Rabbit monoclonal | 1:1,000 for WB |
| Cleaved PARP (Asp214) | Cell Signaling Technology (#9541) | Rabbit monoclonal | 1:2,000 for WB |
| B-actin | Proteintech (60008-1-Ig) | Mouse monoclonal | 1:5,000 for WB |
| HRP-linked anti-rabbit IgG | Cell Signaling Technology (#7074) | 1:3,000 for WB | |
| HRP-linked anti-mouse IgG | Cell Signaling Technology (#7076) | 1:3,000 for WB |
WB, western blot analysis; IF; immunofluorescence; RIP, RNA immunoprecipitation.