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. 2019 Sep 17;75(1):117–125. doi: 10.1093/jac/dkz378

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© The Author(s) 2019. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Appearance of P. aeruginosa biofilms following tobramycin and DEA-C3D treatments, alone and in combination. Biofilms formed by P. aeruginosa clinical CF isolate PA68 were treated with 4 mg/L tobramycin (b), 256 μM DEA-C3D potassium salt (c) or DEA-C3D/tobramycin combination (d), and compared with untreated control biofilms (a). Biofilms were stained with SYTO9 (green=viable cells) and propidium iodide (red=dead/damaged cells) before CLSM. Representative 3D images of each treatment are shown; x- and y-axes=246 μm by 246 μm. Experiments were carried out in duplicate and showed similar results.