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. 2019 Sep 17;75(1):117–125. doi: 10.1093/jac/dkz378

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© The Author(s) 2019. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — DEA-C3D enhances the activity of colistin against P. aeruginosa biofilms. Biofilms formed by clinical CF isolate PA68 were treated with 16 mg/L colistin (b), 256 μM DEA-C3D potassium salt (c) or DEA-C3D/colistin combination (d), and stained with SYTO9 (green=viable cells) and propidium iodide (red=dead/damaged cells) before imaging by CLSM. Untreated control biofilms are shown in (a). Representative 3D images for each treatment are shown; x- and y-axes=246 μm by 246 μm. Experiments were carried out in duplicate and showed similar results.