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. 2019 Dec 2;56(1):178–192. doi: 10.3892/ijo.2019.4928

Figure 7.

Figure 7

Transcription of miR-21 is activated by p-c-Jun. (A) Chromatin immunoprecipitation analysis demonstrated that p-c-Jun binds specifically to the pri-miR-21 promoter region. PCR amplification of the region containing the p-c-Jun recognition sequence in the pri-miR-21 DNA. (B) Inhibition of miR-21 expression by a decrease in c-Jun expression in A498 and 786-O cells. Transfection of A498 and 786-O cells with mock, NC or c-Jun siRNA for 24 h. c-Jun and miR-21 expression levels were examined using reverse transcription-quantitative PCR analysis and normalized to GAPDH and U6 snRNA expression, respectively. Data are presented as the mean ± standard error of the mean. **P<0.01. p-, phospho; miRNA, microRNA; siRNA, small interfering RNA; NC, negative control; IgG, immunoglobulin.