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. 2019 Nov 13;56(1):85–100. doi: 10.3892/ijo.2019.4910

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Copyright: © Yao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Increased SPRY4-IT1 expression results in reduced miR-101 expression in OS cells. (A) Expression of SPRY4-IT1 was significantly upregulated in OS cells compared with the normal osteoblast cells. (B) Expression of miR-101 was significantly downregulated in OS cells compared with normal osteoblast cells. (C) Transfection of shSPRY4-IT1 significantly reduced SPRY4-IT1 levels in MG-63 and U2OS cells. (D) SPRY4-IT1 knockdown resulted in upregulation of miR-101 expression levels in OS cells. (E) Transfection with miR-101 mimics significantly increased miR-101 expression levels. (F) Transfection of miR-101 mimics did not affect SPRY4-IT1 expression. Data are presented as the mean ± standard deviation of three independent experiments. ^**P<0.01, ^***P<0.001. n.s. not significant; SPRY4-IT1, sprouty receptor tyrosine kinase signalling antagonist 4-intronic transcript 1; miR, microRNA; OS, osteosarcoma; sh, short hairpin; NC, negative control.