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. 2019 Nov 13;56(1):85–100. doi: 10.3892/ijo.2019.4910

Figure 4.

Figure 4

SPRY4-IT1 and miR-101 modulate the expression of ZEB1 and ZEB2 in OS cells. shSPRY4-IT1 significantly decreased the mRNA expression levels of (A) ZEB1 and (B) ZEB2 in MG-63 and U2OS cells. miR-101 overexpression significantly decreased the mRNA expression levels of (C) ZEB1 and (D) ZEB2 in MG-63 and U2OS cells. (E and F) Western blotting demonstrated that SPRY4-IT1 knockdown decreased ZEB1 and ZEB2 protein expression levels in MG-63 and U2OS cells. (G and H) Western blotting demonstrated that miR-101 overexpression decreased ZEB1 and ZEB2 protein expression levels in MG-63 and U2OS cells. Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01. SPRY4-IT1, sprouty receptor tyrosine kinase signalling antagonist 4-intronic transcript 1; miR, microRNA; OS, osteosarcoma; sh, short hairpin; NC, negative control; ZEB, zinc finger E-box-binding homeoboxes.