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. 2019 Nov 13;56(1):85–100. doi: 10.3892/ijo.2019.4910

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Copyright: © Yao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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SPRY4-IT1 knockdown exhibits anti-tumour effects in a xenograft mouse model of OS. (A) Xenograft tumours of OS cell lines stably expressing shNC or shSPRY4-IT1. (B) SPRY4-IT1 knockdown significantly reduced tumour volume after 30 days. (C) SPRY4-IT1 knockdown significantly reduced tumour weight in vivo. (D) SPRY4-IT1 levels were significantly lower in the shSPRY4-IT1 xenograft tumour tissues compared with the scrambled control xenograft tumours. (E) miR-101 levels were significantly increased in shSPRY4-IT1 xenograft tumour tissues compared with the scrambled control tumours. (F) ZEB1 mRNA expression levels were significantly lower in the shSPRY4-IT1 xenograft tumour tissues containing shSPRY4-IT1 compared with the scrambled control tumours. (G) ZEB2 mRNA expression levels were significantly lower in shSPRY4-IT1 xenograft tumour tissues compared with the scrambled control tumours. (H and I) ZEB1 and ZEB2 protein expression levels were significantly lower, whereas E-cadherin protein levels were significantly higher, in the shSPRY4-IT1 xenograft tumour tissues compared with the scrambled control tumours. Data are presented as the mean ± standard deviation of three independent experiments. ^*P<0.05, ^**P<0.01. SPRY4-IT1, sprouty receptor tyrosine kinase signalling antagonist 4-intronic transcript 1; miR, microRNA; OS, osteosarcoma; sh, short hairpin; NC, negative control; ZEB, zinc finger E-box-binding homeoboxes.