Extended Data Table 2.

Antibodies for Bone Histology

Fate-mapping models: Flt3Cre;Rosa26LSL-YFP,Cfs1rCre;Rosa26LSL-YFP Csf1rMeriCreMer;Rosa26LSL-YFP and Tnfrsf11aCre;Rosa26LSL-YFP
		Conjugation	Company	Catalog number	Concentration	Dilution	Staining Buffer	Incubating conditions
Primary Antibody	Goat polyclonal anti-GFP	Biotin	Abcam	ab6658	1mg/mL	1::50	PBS/0.25%BSA/0.3% triton	4°C overnight (15hrs)
Secondary Antibody	Streptavidin	AlexaFluor488	Thermo Fisher Scientific	S-11223		1::100	PBS/0.25%BSA	2hrs at room temperature
Parabiosis
		Conjugation	Company	Catalog number	Concentration	Dilution	Staining Buffer	Incubating conditions
Primary Antibodies	Goat polyclonal anti GFP Rabbit polyclonal anti-RFP	Biotin Unconugated	Abcam	ab6658 ab62341	1mg/mL	1::50 1::200	PBS/0.25%BSA/0.3% triton	4°C overnight (15hrs)
Secondary Antibodies	Streptavidin Goat anti-rabbit IgG	AlexaFluor488 AlexaFluor555	Thermo Fisher Scientific	S-11223 A21430	1mg/mL 2mg/mL	1::100 1::200	PBS/0.25%BSA	2hrs at room temperature
Bone marrow monocyte transfusions and transplants
		Conjugation	Company	Catalog number	Concentration	Dilution	Staining Buffer	Incubating conditions
Primary Antibodies	Goat polyclonal anti GFP Rabbit polyclonal anti-RFP	Biotin Unconugated	Abcam Rockland	ab6658 600–401-379	1mg/mL	1::50 1::200	PBS/0.25%BSA/0.3% triton	4°C overnight (15hrs)
Secondary Antibodies	Streptavidin Goat anti-rabbit IgG	AlexaFluor488 AlexaFluor555	Thermo Fisher Scientific	S-11223 A21430	1mg/mL 2mg/mL	1::100 1::200	PBS/0.25%BSA	2hrs at room temperature
EdU staining*
		Conjugation	Company	Catalog number	Concentration	Dilution	Staining Buffer	Incubating conditions
Primary Antibody	Rat anti-mouse Tubulin	Unconjugated	Abcam	ab6160	1mg/mL	1::200	PBS/0.25%BSA/0.3% triton	4°C overnight (15hrs)
Secondary Antibody	Goat anti-rat IgG	AlexaFluor555	Thermo Fisher Scientific	A21434	2mg/mL	1::200	PBS/0.25%BSA	2hrs at room temperature

^*After antibody staining sections were stained with the Click-it EdU Alexa Fluor 488 Imaging Assay kit, as described in methods.