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. 2019 Dec 2;56(1):139–150. doi: 10.3892/ijo.2019.4931

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Copyright: © Yuan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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UNC5C activates integrin α6-associated signaling through interactions with integrin α6. (A) UNC5C interacts with integrin α6 exogenously. 293T cells were co-transfected with a UNC5C-Myc plasmid and integrin α6-Flag plasmid. Immunoprecipitation was performed with MYC and Flag antibody separately. (B) Immunoprecipitation was performed with antibodies against UNC5C and integrin α6 in SK-BR-3 cells for the endogenous interaction analysis. (C) UNC5C interacts with integrin α6 through an extracellular domain. 293T cells were transfected with UNC5C-Myc, integrin α6-FL-Flag, integrin α6-EC-Flag and integrin α6-IC-Flag in the indicated combinations. Immunoprecipitation was performed to precipitate MYC. (D) Western blotting. UNC5C-knockdown exerted no significant effects on integrin α6 in SK-BR-3 cells, decreased ILK phosphorylation, and increased FAK and SRC phosphorylation. Whereas UNC5C-overexpression upregulated integrin α6 expression, increased ILK phosphorylation and decreased FAK and SRC phos-phorylation. FL, full length; EC, extracellular; IC, intracellular; WB, western blot; UNC5C, Unc-5 Netrin Receptor C; ITGA6, integrin α6; p, phosphorylated; ILK, integrin-linked kinase; sh, short hairpin; Ctrl, control; IP, immunoprecipitate; Ab, antibody.