REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rabbit anti-FLAG polyclonal antibody | Sigma | Cat# SAB4301135 RRID# AB_2811010 |
Goat anti-Rabbit IgG antibody, peroxidase conjugated | Sigma | Cat# AP132P RRID# AB_90264 |
Bacterial and Virus Strains | ||
Bacterial Strains, see Table S2 | This paper | N/A |
Phage Strains, see Table S3 | This paper | N/A |
Chemicals, Peptides, and Recombinant Proteins | ||
iQ SYBR Green Supermix | Bio-Rad | Cat# 1708880 |
CSPD™ Substrate (0.25 mM Ready-To-Use) Southern AP Substrate | ThermoScientific | Cat# T2141 |
Clarity Western ECL Substrate | Bio-Rad | Cat# 1705060 |
GelGreen | Biotium | Cat# 41005 |
GelRed | Biotium | Cat# 41003 |
BsaI | New England Biolabs | Cat# R0535S |
BseRI | New England Biolabs | Cat# R0581S |
T4 Ligase | New England Biolabs | Cat# M0202S |
EcoRV-HF | New England Biolabs | Cat# R3195S |
SalI-HF | New England Biolabs | Cat# R3138S |
Critical Commercial Assays | ||
DNeasy Blood & Tissue Kit | Qiagen | Cat# 69506 |
Nano-Glo Luciferase Assay System | Promega | Cat# N1110 |
DIG-High Prime DNA Labeling and Detection Started Kit I | Sigma | Cat# 11745832910 |
TGX Stain-Free FastCast Acrylamide Kit | Bio-Rad | Cat# 1610183 |
Deposited Data | ||
ICP1 _2017_F_Mathbaria | This paper | Deposited at GenBank: accession number MN419153 |
Escape phages | This paper | Deposited in the Sequence Read Archive (SRA) under BioProject ID PRJNA563933 |
Oligonucleotides | ||
Primers used in this study, see Table S4 | This paper | N/A |
Recombinant DNA | ||
Plasmids used in this study, see Table S5 | This paper | N/A |
Software and Algorithms | ||
SPAdes V3.11.1 | (Bankevich et al. 2012) | http://cab.spbu.ru/software/spades/ |
Breseq v0.33.0 | (Deatherage & Barrick 2014) | https://github.com/barricklab/breseq/releases/tag/v0.33.0 |
MUSCLE v3.8.31 | (Edgar 2004) | https://www.drive5.com/muscle/ |
Clustal X v2.0 | (Larkin et al. 2007) | http://www.clustal.org/clustal2/ |
Praline | (Bawono & Heringa 2014) | http://www.ibi.vu.nl/programs/pralinewww/ |
BLASTP | N/A | https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins |
Prism GraphPad | N/A | https://www.graphpad.com/scientific-software/prism/ |
CLC Main Workbench 7 | Qiagen | https://www.qiagenbioinformatics.com/products/clc-main-workbench/ |
ImageJ | NIH | https://imagej.nih.gov/ij/ |
PhyML v20120412 | (Guindon et al. 2005) | https://github.com/stephaneguindon/phyml |
Other | ||
Vecteezy | N/A | https://www.vecteezy.com/ |