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. Author manuscript; available in PMC: 2020 Oct 9.
Published in final edited form as: Cell Host Microbe. 2019 Oct 9;26(4):504–514.e4. doi: 10.1016/j.chom.2019.09.006

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit anti-FLAG polyclonal antibody Sigma Cat# SAB4301135
RRID# AB_2811010
Goat anti-Rabbit IgG antibody, peroxidase conjugated Sigma Cat# AP132P
RRID# AB_90264
Bacterial and Virus Strains
Bacterial Strains, see Table S2 This paper N/A
Phage Strains, see Table S3 This paper N/A
Chemicals, Peptides, and Recombinant Proteins
iQ SYBR Green Supermix Bio-Rad Cat# 1708880
CSPD™ Substrate (0.25 mM Ready-To-Use) Southern AP Substrate ThermoScientific Cat# T2141
Clarity Western ECL Substrate Bio-Rad Cat# 1705060
GelGreen Biotium Cat# 41005
GelRed Biotium Cat# 41003
BsaI New England Biolabs Cat# R0535S
BseRI New England Biolabs Cat# R0581S
T4 Ligase New England Biolabs Cat# M0202S
EcoRV-HF New England Biolabs Cat# R3195S
SalI-HF New England Biolabs Cat# R3138S
Critical Commercial Assays
DNeasy Blood & Tissue Kit Qiagen Cat# 69506
Nano-Glo Luciferase Assay System Promega Cat# N1110
DIG-High Prime DNA Labeling and Detection Started Kit I Sigma Cat# 11745832910
TGX Stain-Free FastCast Acrylamide Kit Bio-Rad Cat# 1610183
 
Deposited Data
ICP1 _2017_F_Mathbaria This paper Deposited at GenBank: accession number MN419153
Escape phages This paper Deposited in the Sequence Read Archive (SRA) under BioProject ID PRJNA563933
Oligonucleotides
Primers used in this study, see Table S4 This paper N/A
Recombinant DNA
Plasmids used in this study, see Table S5 This paper N/A
Software and Algorithms
SPAdes V3.11.1 (Bankevich et al. 2012) http://cab.spbu.ru/software/spades/
Breseq v0.33.0 (Deatherage & Barrick 2014) https://github.com/barricklab/breseq/releases/tag/v0.33.0
MUSCLE v3.8.31 (Edgar 2004) https://www.drive5.com/muscle/
Clustal X v2.0 (Larkin et al. 2007) http://www.clustal.org/clustal2/
Praline (Bawono & Heringa 2014) http://www.ibi.vu.nl/programs/pralinewww/
BLASTP N/A https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins
Prism GraphPad N/A https://www.graphpad.com/scientific-software/prism/
CLC Main Workbench 7 Qiagen https://www.qiagenbioinformatics.com/products/clc-main-workbench/
ImageJ NIH https://imagej.nih.gov/ij/
PhyML v20120412 (Guindon et al. 2005) https://github.com/stephaneguindon/phyml
Other
Vecteezy N/A https://www.vecteezy.com/