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. Author manuscript; available in PMC: 2019 Dec 13.
Published in final edited form as: Neuroimaging Clin N Am. 2018 Sep 15;28(4):611–623. doi: 10.1016/j.nic.2018.06.005

FIGURE 8.

FIGURE 8

Neutrophil extracellular traps in ischemic stroke thrombi. Intracranial thrombi retrieved from stroke patients that underwent thrombectomy procedure were collected for histological analysis. Consecutive thrombi sections were stained with H&E and antibodies against citrullinated histones (H3Cit), a marker of NET-formation. Extracellular zones of nuclear material were often observed on H&E stainings (left), which were also positive for H3Cit (right). Scale bars: 10 μm. Right: Fresh thrombi (n=8) retrieved from ischemic stroke patients, were used for ex vivo lysis experiments. The thrombus parts were incubated for 120 min at 37°C in the presence of either t-PA alone (black) or t-PA plus DNase 1 (grey). Thrombus weight (percentage of original weight) was measured at time points 0, 10, 30, 60 and 120 minutes. Data are represented as mean with SD (** p < 0.01).

Adapted from Laridan E, Denorme F, Desender L, et al. Neutrophil extracellular traps in ischemic stroke thrombi. Ann Neurol. 2017; 313:1451–10; with permission.