FIG. 2.

Process for free-energy landscape reconstruction of BR using an inverse Weierstrass transform (IWT). (a) A heat map of all force-extension curves used in this work. Data within ∼18 nm of the surface are excluded due to surface adhesion. (b) Different components for calculating the molecular free energy ΔG₀ as a function of the molecular extension when using the IWT. The three components used in computing ΔG₀ as defined in Eq. (5): A (aqua, dotted-dashed) and terms involving its first and second derivative (pink solid line and brown dashed line, respectively). To promote comparison to panels (a) and (c), the plotted lateral axis is q, molecule extension. (c) The ΔG₀ free energy as a function of molecular extension. Shaded region reports the standard deviation as deduced from a bootstrap analysis.