Table 1.
Gene Amplified |
Sequence |
Annealing Temperature |
T17M forward | GAG TGC ACC CTC CTT AGG CA | 55°C |
T17M reverse | TCC TGA CTG GAG GAC CCT AC | |
mRHO forward | GAT TAG CGT TAG TAT GAT ATC TCG | 52°C |
mRHO reverse | AAG AGA CTC AAG TAA GCT GCA | |
rd8 forward 1 | GTGAAGACAGCTACAGTTCTGATC | 65°C |
rd8 forward 2 | GCCCCTGTTTGCATGGAGGAAACTTGGAAGACAGCTA | |
rd8 reverse | CAGTTCTTCTGGCCCCATTTGCACACTGATGAC | |
rd1 sense | CAT CCC ACC TGA GCT CAC AGA AAG | 55°C |
rd1 antisense | GCC TAC AAC AGA GGA GCT TCT AGC | |
RPE65 sense | CAC TGT GGT CTC TGC TAT CTT C | 55°C |
RPE65 antisense | GGT GCA GTT CCA CTT CAG TT |