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. 2019 Dec 13;14(12):e0226406. doi: 10.1371/journal.pone.0226406

Fig 5. Knockdown of MLCK in cultured coronary arterial smooth muscle cells.

Fig 5

MLCK protein was down-regulated in human CASMC by siRNA treatment. Controls were treated identically, but with negative control siRNA. (A) Representative western blots of two different knockdown experiments demonstrating siRNA-mediated down-regulation of MLCK. (B) Representative western blots showing the lack of effect of MLCK knockdown on ROCK1 or ZIPK expression in CASMC. (C) Quantitative analysis of the effects of MLCK knockdown on MLCK, ROCK1 and ZIPK expression. Statistical analysis was carried out with Dunnett’s post hoc test.