Fig 9. Effect of EGTA treatment on serum-induced LC₂₀ phosphorylation.

CASMC were incubated in medium containing Ca²⁺ (2.5 mM) or EGTA (5 mM) and treated with serum for 2 min prior to SDS-PAGE and western blotting with anti-2P-LC₂₀ (A; left-hand panel) or Phos-tag SDS-PAGE and western blotting with anti-LC₂₀ (B; upper panel). Quantitative data are depicted in the corresponding histograms in panels A and B (n = 3 in panel A and n = 6 in panel B). “ns” denotes p > 0.05 (Student’s unpaired t-test).