Fig 11. Effect of knockdown of ROCK1 or ZIPK on phosphorylation of LC₂₀, MYPT1 and Par-4 in human arterial smooth muscle cells.

ROCK1 or ZIPK was down-regulated in CASMC and UASMC by siRNA treatment. Controls were treated identically, but with negative control siRNA. Cells were serum starved overnight and treated with 5% FBS for 2 min prior to lysis in Laemmli sample buffer for SDS-PAGE and western blotting. GAPDH and actin were used as loading controls; each control shown is for the blot(s) above it. Representative western blots are shown in panels A (CASMC) and B (UASMC) and cumulative quantitative data are provided in the histograms (C, D). Data in panels C and D are presented as % control and values represent the mean ± SEM with the indicated p and n values with p < 0.05 considered statistically significant (Dunnett’s post hoc test).