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. 2019 Oct 24;8:e50471. doi: 10.7554/eLife.50471

Figure 4. RhoGDI is recruited to single-cell wounds enriched in Rho and Cdc42 activity.

(A) Oocytes microinjected with wGBD (magenta), rGBD (blue) and GDI (green); (A’) Kymograph of wound closure and line scan of radially-averaged fluorescence intensity from (A); (B) Quantification of distance between peaks (n = 16); (C) Wounded oocytes fixed and stained with anti-X. laevis GDI. Scale bar 10 μm, time min:sec.

Figure 4—source data 1. RhoGDI is recruited to single-cell wounds enriched in Rho and Cdc42 activity.

Figure 4.

Figure 4—figure supplement 1. X. laevis RhoGDI antibody specificity.

Figure 4—figure supplement 1.

Western blot stained with αGDI antibody to determine specificity; lane 1: X. laevis oocyte whole cell lysate (WCL), lane 2: WCL of oocytes overexpressing GDI, lane 3: WCL of oocytes expressing 3xGFP-GDI, lane 4: purified FLAG-GDI.