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. 2019 Dec 2;12:10535–10545. doi: 10.2147/OTT.S210059

Figure 4.

Figure 4

CBX4 is responsible for miR-497-5p regulated cervical cancer proliferation. (A and B) qRT-PCR (A) and Western blot (B) examination for CBX4 expression in Siha and HeLa cells treated with miR-497-5p inhibitors (miR-497-in) and/or CBX4 interference RNAs (SiCBX4-1, SiCBX4-2). (C) MTT examination for Siha and HeLa cells’ proliferation. (D) Flow cytometry examination for Siha and Hela cells, cell cycle changes (left). Cells pre-transfected with different RNAs for 48 hrs were used for above examination. Right is a statistical result of three independent experiments. (E) Western blot examination for CDK2 and Cyclin A2 expression. Siha and HeLa cells treated like A were protein extracted and used for Western blot test. Bottom is the relative blot change of the gray value. For all data, * means P< 0.05, ** means P<0.01, *** means P<0.001.