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. 2019 Dec 13;10:5697. doi: 10.1038/s41467-019-13587-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Levels of Tet2 mRNA after TCR stimulation of CD4⁺ T cells isolated from lymph nodes of BALB/c mice, as revealed by qPCR normalized to Histone mRNA, n ≥ 8. b Levels of Tet2 mRNA after TCR stimulation of CD4⁺ T cells as described in a, in presence of miR142-3p inhibitor, as revealed by qPCR normalized to Histone mRNA, n = 5. c Tet2 protein abundance after TCR stimulation of CD4⁺ T cells as described in a, as revealed by flow cytometry, n = 5. d Tet2 protein abundance after TCR stimulation of CD4⁺ T cells as described in a, in presence of miR142-3p inhibitor, as revealed by flow cytometry, n = 6. e Normalized luciferase activity of HEK-293 cells cotransfected with miR142-3p mimic and wild-type TET2 3′UTR reporter construct, n = 4. f Levels of Tet2 mRNA in 3T3 fibroblasts transfected with miR142-3p mimic, as revealed by qPCR normalized to Histone mRNA, n = 6. g Levels of Tet2 mRNA after TCR stimulation of CD4⁺ T cells isolated from lymph nodes of miR142^+/+ and miR142^-/- mice, as revealed by qPCR normalized to Histone mRNA, n = 4. h Tet2 protein abundance after limited TCR stimulation of CD4⁺ T cells as described in g, as revealed by flow cytometry, n = 6. i In vitro Treg induction in presence of Tet2 siRNA, using naive CD4⁺ T cells isolated from lymph nodes of BALB/c mice, as revealed by flow cytometry, n = 6. j In vitro Treg induction in presence of Tet2 siRNA, using naive CD4⁺ T cells isolated from human peripheral blood, as revealed by flow cytometry, n = 4. One data point represents one subject. Experiments were performed in three technical replicates per subject. Data are presented as box-and-whisker plots with mean, 25% percentile, 75% percentile, minimum and maximum values or as means ± s.e.m. (a, c, e) Ordinary one-way ANOVA, Tukey’s multiple comparisons test. (b, d, f–j) Student’s t-test, *P < 0.05, **P < 0.01, and ***P < 0.001. The source data are provided as a Source Data file.