Table 1.
Results of peak area ratio (2PA-d6/ 2PA) (n = 3; mean ± standard deviation, %).
| Analyte |
m/z (2PA) |
m/z (2PA-d6) |
Retention Time (min) |
Std. mixture | Human plasma | Mouse plasma | QC plasma (human) |
QC plasma (mouse) |
|---|---|---|---|---|---|---|---|---|
| Propanoic acid | 165.1 | 171.1 | 2.5 | 0.95 ± 0.11 | 0.92 ± 0.04 | 0.98 ± 0.04 | 0.88 ± 0.04 | 0.87 ± 0.01 |
| Butyric acid | 179.1 | 185.1 | 5.1 | 0.92 ± 0.13 | 0.87 ± 0.04 | 1.04 ± 0.11 | 0.83 ± 0.07 | 0.88 ± 0.02 |
| Indole-3-acetic acid | 266.1 | 272.2 | 11.6 | 0.92 ± 0.16 | 1.33 ± 0.13 | 1.37 ± 0.10 | 1.25 ± 0.17 | 1.16 ± 0.15 |
| Lauric acid | 291.1 | 297.2 | 18.6 | 0.99 ± 0.03 | 0.96 ± 0.03 | 1.29 ± 0.18 | 0.91 ± 0.12 | 1.02 ± 0.09 |
| Stearidonic acid | 367.3 | 373.1 | 19.9 | 1.27 ± 0.07 | 1.18 ± 0.02 | 1.09 ± 0.14 | 1.17 ± 0.13 | 1.22 ± 0.25 |
| Eicosapentaenoic acid | 393.2 | 399.3 | 21.1 | 1.37 ± 0.06 | 1.09 ± 0.03 | 0.99 ± 0.17 | 1.10 ± 0.17 | 1.14 ± 0.30 |
| α-Linolenic acid | 369.3 | 375.2 | 21.2 | 1.37 ± 0.04 | 1.12 ± 0.05 | 0.94 ± 0.15 | 1.12 ± 0.18 | 1.10 ± 0.25 |
| Myristic acid | 319.3 | 325.3 | 21.3 | 0.96 ± 0.05 | 1.03 ± 0.12 | 0.95 ± 0.11 | 0.84 ± 0.19 | 0.80 ± 0.19 |
| γ-Linolenic acid | 369.3 | 375.2 | 21.5 | 1.28 ± 0.05 | 1.08 ± 0.03 | 0.89 ± 0.16 | 1.06 ± 0.18 | 1.06 ± 0.27 |
| Palmitoleic acid | 345.3 | 351.2 | 22.0 | 1.46 ± 0.03 | 1.18 ± 0.05 | 1.04 ± 0.14 | 1.19 ± 0.22 | 1.20 ± 0.26 |
| Docosahexaenoic acid | 419.3 | 425.4 | 22.5 | 1.20 ± 0.06 | 0.84 ± 0.06 | 0.74 ± 0.06 | 0.85 ± 0.17 | 0.86 ± 0.27 |
| Arachidonic acid | 395.3 | 401.3 | 22.8 | 1.21 ± 0.06 | 1.10 ± 0.04 | 0.97 ± 0.06 | 1.11 ± 0.25 | 1.12 ± 0.35 |
| Linoleic acid | 371.3 | 377.3 | 22.9 | 1.05 ± 0.03 | 0.93 ± 0.04 | 0.90 ± 0.11 | 0.93 ± 0.20 | 0.91 ± 0.24 |
| Docosapentaenoic acid | 421.3 | 427.4 | 23.3 | 1.06 ± 0.07 | 0.78 ± 0.06 | 0.74 ± 0.13 | 0.78 ± 0.20 | 0.77 ± 0.26 |
| Dihomo-γ-Linolenic acid | 397.3 | 403.4 | 24.0 | 1.46 ± 0.03 | 1.10 ± 0.09 | 0.88 ± 0.08 | 1.13 ± 0.28 | 1.06 ± 0.35 |
| Palmitic acid | 345.3 | 351.2 | 24.4 | 0.96 ± 0.10 | 0.90 ± 0.09 | 0.78 ± 0.07 | 0.92 ± 0.27 | 0.81 ± 0.18 |
| Adrenic acid | 423.4 | 429.4 | 25.0 | 0.92 ± 0.12 | 0.69 ± 0.08 | 0.61 ± 0.10 | 0.70 ± 0.19 | 0.63 ± 0.24 |
| Oleic acid | 373.1 | 379.4 | 25.1 | 1.11 ± 0.10 | 0.79 ± 0.06 | 0.71 ± 0.11 | 0.82 ± 0.22 | 0.74 ± 0.21 |
| Stearic acid | 375.3 | 381.4 | 27.6 | 1.04 ± 0.07 | 1.13 ± 0.29 | 0.83 ± 0.07 | 1.16 ± 0.43 | 0.91 ± 0.24 |
| Arachidic acid | 403.4 | 409.4 | 29.6 | 1.39 ± 0.16 | 1.20 ± 0.27 | 0.83 ± 0.04 | 1.26 ± 0.45 | 0.94 ± 0.23 |
| Nervonic acid | 457.4 | 463.5 | 30.3 | 1.02 ± 0.08 | 0.72 ± 0.09 | 0.60 ± 0.11 | 0.71 ± 0.18 | 0.62 ± 0.15 |
| Lignoceric acid | 459.4 | 465.5 | 31.1 | 1.20 ± 0.28 | 0.75 ± 0.28 | 0.82 ± 0.04 | 0.88 ± 0.29 | 0.79 ± 0.11 |
| Glyco-α/β-muricholic acid | 556.6 | 562.6 | 7.3 | 0.88 ± 0.04 | ND | ND | 0.82 ± 0.06 | 0.88 ± 0.03 |
| ω-muricholic acid | 499.5 | 505.5 | 8.7 | 0.91 ± 0.03 | ND | ND | 0.84 ± 0.06 | 0.85 ± 0.01 |
| α-muricholic acid | 499.5 | 505.5 | 9.1 | 1.00 ± 0.05 | ND | ND | 0.88 ± 0.04 | 0.87 ± 0.04 |
| β-muricholic acid | 499.5 | 505.5 | 9.4 | 0.90 ± 0.03 | ND | 0.82 ± 0.12 | 0.85 ± 0.02 | 0.83 ± 0.04 |
| Glycohyocholic acid | 556.6 | 562.6 | 9.4 | 0.95 ± 0.05 | ND | ND | 0.97 ± 0.06 | 0.89 ± 0.07 |
| Glycoursodeoxycholic acid | 540.6 | 546.6 | 11.1 | 1.04 ± 0.06 | ND | ND | 0.90 ± 0.12 | 0.93 ± 0.02 |
| Glycohyodeoxycholic acid | 540.6 | 546.6 | 11.1 | 1.01 ± 0.04 | ND | ND | 0.91 ± 0.12 | 0.93 ± 0.02 |
| Glycocholic acid | 556.6 | 562.6 | 11.3 | 1.04 ± 0.05 | 1.24 ± 0.31 | ND | 1.05 ± 0.13 | 1.00 ± 0.04 |
| Hyocholic acid | 499.5 | 505.5 | 11.5 | 0.91 ± 0.04 | ND | ND | 0.90 ± 0.10 | 0.88 ± 0.04 |
| Ursodeoxycholic acid | 483.5 | 489.5 | 12.1 | 0.95 ± 0.04 | 0.95 ± 0.11 | ND | 0.86 ± 0.04 | 0.87 ± 0.01 |
| Cholic acid | 499.5 | 505.5 | 12.5 | 0.96 ± 0.04 | 0.87 ± 0.06 | 0.79 ± 0.05 | 0.84 ± 0.03 | 0.89 ± 0.04 |
| Hyodeoxycholic acid | 483.5 | 489.5 | 12.6 | 0.84 ± 0.01 | 0.92 ± 0.16 | ND | 0.82 ± 0.03 | 0.84 ± 0.03 |
| Glycochenodeoxycholic acid | 540.6 | 546.6 | 14.1 | 1.08 ± 0.15 | 0.55 ± 0.20 | ND | 0.87 ± 0.10 | 0.71 ± 0.12 |
| Glycodeoxycholic acid | 540.6 | 546.6 | 14.1 | 0.98 ± 0.04 | 0.93 ± 0.04 | ND | 0.91 ± 0.07 | 0.93 ± 0.03 |
| Chenodeoxycholic acid | 483.5 | 489.5 | 14.1 | 0.97 ± 0.05 | 0.88 ± 0.03 | ND | 0.87 ± 0.03 | 0.91 ± 0.02 |
| Deoxycholic acid | 483.5 | 489.5 | 14.8 | 0.97 ± 0.04 | 0.90 ± 0.06 | 0.99 ± 0.10 | 0.92 ± 0.06 | 0.93 ± 0.04 |
| Glycolithocholic acid | 524.6 | 530.6 | 15.3 | 1.03 ± 0.06 | 0.82 ± 0.10 | ND | 0.86 ± 0.07 | 0.89 ± 0.10 |
| Lithocholic acid | 467.5 | 473.5 | 16.0 | 0.90 ± 0.03 | 0.91 ± 0.04 | ND | 0.89 ± 0.08 | 1.00 ± 0.14 |
ND; Non-detected.