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. 2019 Dec 13;9:19082. doi: 10.1038/s41598-019-55663-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The role of Bmal1 overexpression/inhibition and melatonin in the regulation of AKT pathway components after OGD. To analyze PI3K signaling pathway components, cell extracts were prepared and analyzed using PathScan® AKT Signaling Antibody Array Kit. p-AKT (Thr308), p-ERK-1/2 (Thr202/Tyr204), p-mTOR (Ser2481), p-Pdk1 (Ser241), p-PTEN (Ser380), p-Bad (Ser112), p-Gsk-3α (Ser21), p-Gsk-3β (Ser9), (i), Ampkα (Thr172), p-PRAS40 (Thr246), p-4E-BP1 (Thr37/46), p-RSK1 (Thr421/Ser424), p-rp-s6 (Ser235/236), p-p70S6K (Thr421/Ser424) were evaluated for Bmal1 overexpression (a) and Bmal1 inhibition (b). Representative images of planar surface immunoassay analysis were given in the Supplementary Fig. S6. Data are mean ± SD (n = 4/group). **p < 0.01/*p < 0.05 compared with vehicle (empty vector; pAcGFP1-N1 Vector), ^##p < 0.01/^#p < 0.05 compared with Melatonin, ^§§p < 0.01/^§p < 0.05 compared with Bmal1 transfected group for (a) and **p < 0.01/*p < 0.05 compared scrambled RNA (scRNA), ^##p < 0.01 compared with Melatonin, ^§§p < 0.01/^§p < 0.05 compared with shBmal1 group for (b).