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. 2019 Dec 13;9:19045. doi: 10.1038/s41598-019-55659-w

Figure 4.

Figure 4

GFP expression in worms fed (A) E. coli XL1 containing pBac [3xp3-EGFPafm] plasmid; (B) only the pBac [3xp3-EGFPafm] plasmid; (C) 3.2 kb PCR fragment containing complete gpf gene. (D) the bacteria E. coli XL1 harbouring the pBac [3xp3-EGFPafm] plasmid; E1) Stenostomum cell, stained with DAPI, in bright-field microscopy; E2) in fluorescence microscopy (blue = nucleus); F1) Stenostomum cell of worms fed E. coli XL1 containing pBac [3xp3-EGFPafm] plasmid, stained with DAPI, in bright-field microscopy; F2) in fluorescence microscopy (blue = nucleus; green = GFP). Sixty worms were visualized under the microscope in each assay.