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. 2019 Dec 13;9:19045. doi: 10.1038/s41598-019-55659-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of the gfp reporter gene under the control of different promoters. (A) GFP expression mainly in the ciliated pits (CPF, arrowhead) when plasmids have Pax6 regulatory elements in the promotor; (B) pattern of diffuse fluorescence (DF) spread over the body observed in worms expressing mCherry red fluorescent protein under control of the histone H3 promotor; (C) percentage of worms showing diffuse fluorescence spread over the body (blue bars) or in the ciliated pit (green bars), for worms fed with: E. coli carrying 3xp3-EGFPafm (with Pax6 RE) plasmid or only the plasmid (D); (E) PCR fragments containing the GFP gene with Pax6 RE (green bars). Whiskers represent the standard deviation. (F) Transcriptome assay for worms fed 3xp3-EGFPafm plasmid showed reads covering only the gfp gene region.