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. 2019 Dec 13;9:19045. doi: 10.1038/s41598-019-55659-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stable GFP expression induced by the new E. coli strain isolated in nature, harbouring the pBac [3xp3-EGFPafm] plasmid: (A) Ssp-sm09, control - worms fed E. coli without plasmid (A1 = bright-field; A2 = fluorescence microscopy); (B) Ssp-sm09 fed with E. coli harbouring pBac[3xp3-EGFPafm] plasmid (B1 = bright-field; B2 = fluorescence microscopy); (C) Ssp-sm09 fed “naked” pBac[3xp3-EGFPafm] plasmid (C1 = bright-field; C2 = fluorescence microscopy); (D) microbiome present in the Ssp-sm09 strain. The bar plots represent relative abundance (in %).