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. 2019 Dec 13;9:19111. doi: 10.1038/s41598-019-55361-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of holding potential on peak current response and pEC₅₀ values. Illustrative current-voltage (I/V) data from two different oocytes that responded with low (a,c,e) and high (b,d,f) maximal peak current responses. (a,b) Current-voltage (I/V) plots at different 5-HT concentrations (■ 10 µM; △ 6 µM; + 3 µM; ● 2 µM; ⬦ 1 µM; ▲ 0.3 µM; × 0.1 µM). (c, d) Peak current shown as a function of 5-HT concentration at differing holding potentials (−80 to + 40 mV in 10 mV steps). The fitted curves represent the Partial Model in which pEC₅₀ and n_H parameters are constrained to be the same across all holding potentials for each oocyte. The pEC₅₀ is indicated by the dotted line. (e, f) The relationship between the fitted parameters, I_max, pEC₅₀ and n_H, and the holding potential. The values shown are from the Full Model in which the parameters can adopt different values for each holding potential. The figures illustrate the variance in the unconstrained estimates of pEC₅₀ and n_H. Likelihood Ratio Tests comparing the Full and Partial Models suggest that there is no difference in the pEC₅₀ and n_H values between different holding potentials (Low responder: P = 0.74; High responder: P = 0.93). The straight line gradients (mean ± SE) of the data in Panel (e) are: I_max, 0.031 ± 0.001 (P = 3 × 10^-11); pEC₅₀, −0.00051 ± 0.00025 (P = 0.063); n_H, −0.0100 ± 0.0062 (P = 0.14), and in Panel (f) are: I_max, 0.17 ± 0.0004 (P = 5 × 10^-24); pEC₅₀, −0.00015 ± 0.00029 (P = 0.62); n_H, −0.0013 ± 0.0045 (P = 0.78). Note the differing y axis scales in the panels.