Table 1.

Genes affecting C-circles formation in either FANCM proficient or FANCM deficient ALT cells. The targeted screening was done using the same CC assay as describe in the methods. The values shown are the mean obtained from at least two independent experiments. ± : standard deviation. Standard two-tailed Student’s t-test: *p < 0.05, ns – no significance.

	FANCM Proficient	FANCM Deficient
Control (Luc)	100	100
Dissolvase
BLM	41 ± 4.2(*)	18.5 ± 4.9(*)
TOP3A	77 ± 4.2(ns)	41 ± 2.2(*)
RMI1	88 ± 3.5(ns)	64 ± 1.4(*)
Checkpoint kinase
ATR	153 ± 4.9(*)	62 ± 2.1(*)
Chk1	145 ± 2.1(*)	17 ± 2.8(*)
Break-induced replication
PolD3	88 ± 12(ns)	34 ± 5.7(*)
DNA polymerase
PolD1	115 ± 7.1(ns)	35 ± 7.1(*)
PolH	102 ± 12(ns)	25.5 ± 7.8(*)
Homologous recombination
BRCA1	110 ± 11.3(ns)	102 ± 15.6(ns)
BRCA2	237 ± 9.2(*)	53 ± 1.3(*)
PALB2	124 ± 4.9(ns)	62 ± 1.4(*)
Rad51	89 ± 2.1(ns)	46 ± 2.8(*)
Rad52	106 ± 14(ns)	98 ± 10.6(ns)
DNA end resection
CtIP	92 ± 2.1(ns)	104 ± 11.3(ns)
Mre11	249 ± 12(*)	105 ± 21.9(ns)
Cohesion-like complex
Smc5	194 ± 7.1(*)	87 ± 6(ns)
Smc6	264 ± 4.9(*)	88 ± 7.8(ns)
Others
FANCD2-1	226 ± 8.5(*)	43 ± 4.2(*)
FANCD2-2	175 ± 7.1(*)	26 ± 5.7(*)