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. 2019 Dec 13;9:19028. doi: 10.1038/s41598-019-54413-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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V-type H⁺-ATPase (VA) immunolocalization in the rectum (RM) of wild-collected A. aegypti larvae reared in freshwater (FW) and septic water (Septic). VA immunostaining (green) in representative paraffin-embedded cross sections of the (a) RM and Malpighian tubules (MT) from FW-reared wild A. aegypti larvae and (b) RM of septic-reared wild A. aegypti larvae. Nuclei are labelled with DAPI (blue). (c) cross section of MT and RM from (a) with apical AeRh50 staining (red) showing colocalization of with VA in MT (merge, yellow) in FW larvae and (d) cross section of RM corresponding to (b) showing co-localization (merge, yellow) of VA with apical AeRh50 staining (red) in septic larvae. Scale bars: 50 µm. Lumen (lm); rectum (RM), Malpighian tubule (MT).