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. 2019 Dec 13;9:19028. doi: 10.1038/s41598-019-54413-6

Figure 5.

Figure 5

Scanning ion-selective micro-electrode technique (SIET) measurements of NH4+ flux at the anal papillae of wild-collected and laboratory A. aegypti larvae reared in freshwater (FW) and septic water (Septic). (A) NH4+ flux at the anal papillae of wild A. aegypti larvae reared in FW or Septic (n = 4 for FW, n = 6 for Septic), and (B) NH4+ flux at the anal papillae of laboratory A. aegypti larvae reared in FW of Septic (n = 5 for FW and Septic), measured each in their respective freshwater or septic water baths. NH4+ flux for each individual animal is shown as a single point, with the mean flux for each group illustrated by a horizontal solid black line. Negative values indicate efflux, or excretion, and positive values indicate influx, or absorption from the external. Data shown as mean ± S.E.M. Asterisks indicate statistical significance (*p < 0.05) compared to FW control (Unpaired, two-tailed t-test).