Fig. 4. Elba and Insv repress target gene expression.

a Luciferase reporter assays with the TetR-DNA-binding domain (DBD) fusion proteins. The Elba and Insv proteins were fused with the TetR-DBD and thus brought to the 2 × Tet operator sites in the luciferase reporter. The values of firefly/renilla luciferase ratio are plotted and represent the average from three biological replicates with each biological replicate having four technical replicates. So 12 values are included for each condition (n = 12), and error bars represent S.D. p-value is calculated using a Student’s t test, two-tailed, type 1. ***p < 0.001. Source data are available in a Source Data file. b The four bar plots show log₂ fold change of RNA level of target genes in the mutant versus wt based on RNA-seq data. For each factor, the top 200 bound genes that contain the Elba/Insv motifs (red) were more upregulated than all the target genes with the Insv/Elba motifs (green) and the target genes without the Insv/Elba motifs (black). Pairwise statistical significance among the three sets were calculated by two-tailed t tests, and p-values were adjusted by the Bonferroni multiple testing correction method (*p < 0.05, **p < 0.01, ***p < 0.001, n.s. not significant).