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. 2019 Dec 13;9:19102. doi: 10.1038/s41598-019-55617-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of HIPP1 protein to genomic sites in pupae from transgenic lines expressing full-length Su(Hw)⁺, Su(Hw)^Δ283, and Su(Hw)^Δ114. (a) SBS-CM sites; (b) SBS-C sites; (c) SBS-O sites; (d) CP190 alone sites. Su(Hw)⁻ refers to the y² w¹¹¹⁸; su(Hw)^v/su(Hw)^e04061 background. The different variants of the Su(Hw) protein were expressed in the y²sc^D1ct⁶; P{Su(Hw)}-38D/P{Su(Hw)}-38D; su(Hw)^v/su(Hw)^e04061 lines, where P{Su(Hw)} are Su(Hw)⁺ − P{w⁺; UbqW-Su(Hw)1-945-FLAG}; Su(Hw)Δ114 − P{w⁺; UbqW-Su(Hw)1-88/202-945-FLAG}; Su(Hw)Δ283 − P{w⁺; UbqW-Su(Hw)1-760/778-945-FLAG}. The ras64B coding region (ras) was used as a control devoid of Su(Hw) binding sites. The percent recovery of immunoprecipitated DNA (Y-axis) was calculated relative to the amount of input DNA. Error bars indicate standard deviation of three independent biological replicates. Asterisks indicate significance levels (Student’s t-test) of *p < 0.05, **p < 0.01, or ***p < 0.001. P values are not displayed for significance >0.05. Significance levels are shown for treatment comparisons indicated by horizontal brackets (here and in Figs. 4–6). ChIP was performed with antibodies against HIPP1. Statistical analysis was performed relative to the Su(Hw)+ variant.