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. 2019 Dec 13;9:19102. doi: 10.1038/s41598-019-55617-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of HIPP1 and insulator proteins to SBS-CMs in pupae from lines bearing mod(mdg4)^u1 and Hipp^Δ1 mutations. ChIP was performed with antibodies against (a) HIPP1; (b) Mod(mdg4)-67.2; (c) the Su(Hw) N-terminal domain; and (d) CP190. The lines used in this analysis are designated as [wt] − y²w¹¹¹⁸, [mod⁻] − y²w¹¹¹⁸, mod(mdg4)^u1/mod(mdg4)^u1, [HIPP1⁻] − y²w¹¹¹⁸;Hipp^Δ1/Hipp^Δ1, and [HIPP1⁻ mod⁻] − y²w¹¹¹⁸;Hipp^Δ1mod(mdg4)^u1/Hipp^Δ1mod(mdg4)^u1. Statistical analysis was performed relative to the wild type condition. Other designations are as described in Fig. 2.