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. 2019 Dec 13;9:19100. doi: 10.1038/s41598-019-55615-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CD36 and CD27 on HIV-1 virions in blood plasma of infected individuals. (a) Gating strategy: threshold trigger setting on fluorescence height of MNP label Zenon Alexa 647. (b) Single gate defined by fluorescence height and fluorescence width, which excludes aggregates¹². (c) Flow virometry of 2G12/PG9-captured HIV-1. Populations of CD27⁺ and CD36⁺ virions are presented. (d,e) CD27⁺ and CD36⁺ on HIV-1 virions captured with 2G12/PG9-MNPs (white asterisks). CD27 (red arrows) (d) and CD36 (green arrows) (e) are revealed by 6-nm colloidal gold particles. (f) Fractions of CD36⁺ and CD27⁺ among 2G12/PG9-captured virions as evaluated with flow virometry. Presented are means ± s.e.m. (n = 3). (g) HIV-1 RNA in fractions of virions captured with 2G12/PG9-MNPs, anti-CD36-MNPs, and anti-CD27-MNPs among total blood plasma virions. Presented are means ± s.e.m. (n = 3).