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. 2019 Dec 13;10(12):951. doi: 10.1038/s41419-019-2185-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a WB from both untreated and irradiated mocked and siNBS1– MRC5 cell extracts. Cells were irradiated with 5 Gy of X-rays and harvested after 0.5 h. Ten micrograms of total protein lysates were analyzed by WB. Membranes were probed with anti-HP1α and anti-NBS1 antibodies; vinculin and β-actin were used as loading controls. b WB from extracts of lymphoblastoid cells (LCLs) established from an healthy donor (Ctrl) and from a patient carrying two germline mutations in the RAD50 gene (RAD50^−/−)³⁰. Membranes were probed with anti-HP1α, anti-NBS1, and anti-RAD50 antibodies; β-actin was used as loading control. c Left: representative images of the immunofluorescence analysis of HP1α (Alexa Fluor 480, green fluorophore green) protein levels in mocked and NBS1-silecenced MRC5 cells. Bar: 20 μm. Right: Distribution of HP1α corrected nuclear fluorescence intensity in mocked and NBS1-silecenced MRC5 cells. The horizontal lanes indicate the mean values derived from the analysis of 100 cells/experimental point in three independent experiments ±S.D. (t Student’s Test; ***p < 0.001).