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. 2019 Dec 13;10(12):951. doi: 10.1038/s41419-019-2185-x

Fig. 7. NBS1 fragments arising from the 657del founder mutation in NBS1 patients increase HP1α levels.

Fig. 7

a WB analysis from total protein lysate derived from NBS cells, immunoprecipitated with either anti-HP1α or with two different anti-NBS1 antibodies directed against the N- and the C-terminus of NBS1. Membranes were probed with the anti-HP1α or the two anti-NBS1 antibodies. The corresponding inputs (1%), probed with anti-HP1α and anti-vinculin antibodies, are shown below. b One milligram of whole-protein lysate obtained from NBS1 cells were incubated in the absence (−) or presence (+) of 10 U alkaline phosphatase (AP). The untreated and dephosphorylated samples were immunoprecipitated with anti-HP1α antibody and membranes were probed with anti-NBS1 antibody directed against the C-terminus of NBS1. Total IgG levels and vinculin were used as loading controls for immunoprecipitates and input, respectively.