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. 2019 Dec 13;10:5696. doi: 10.1038/s41467-019-13639-8

Fig. 5. Fast CORT feedback has little impact on CRH neural activity but faithfully suppresses ACTH release.

Fig. 5

a Mean photometry signals of CRH neuron activity during WN stress, 30 min after injection of either vehicle or CORT. b Heatmap of mean CRH neuron activity from all individual mice in 10 s bins. c Average ΔF/F across 5 min of CRH neuron activity before, during, and after WN; n = 12 per group, RM two-way ANOVA, ***p < 0.001 vs. baseline, Holm–Sidak; ANOVA interaction p = 0.19, ANOVA main effect of group p = 0.14. d Peak ΔF/F at WN onset; Mann–Whitney test. e Cumulative integrated ΔF/F from the time of WN stress; RM two-way ANOVA, *p < 0.05 Veh vs. CORT, Holm–Sidak; ANOVA interaction p < 0.001, ANOVA main effect of group p = 0.21. Lines indicate points at which statistical significance was reached and its duration. Gray shaded area indicates duration of WN. f Plasma ACTH levels 5 min post WN; one-way ANOVA, Tukey’s multiple comparison test. All data presented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.