Table 1. Spearman’s correlation coefficients (rs): qPCR (A,C) and TCGA RNA-Seq data (B) in NSCLC (ADC, SCC).
| A | RB1 | CTDSP1 | CTDSP2 |
|---|---|---|---|
| Lung ADC (qPCR) | |||
| CTDSPL | 0.67 (P=0.013) | 0.67 (P=0.035) | 0.55 (P=0.4) |
| RB1 | – | 0.54 (P=0.1) | 0.20 (P=0.3) |
| CTDSP1 | – | – | 0.42 (P=0.2) |
| Squamous cell lung cancer (qPCR) | |||
| CTDSPL | 0.74 (P<0.001) | 0.50 (P=0.003) | 0.73 (P<0.001) |
| RB1 | 0.31 (P=0.07) | 0.65 (P<0.001) | |
| CTDSP1 | – | – | 0.73 (P<0.001) |
| B | CTDSPL | CTDSP1 | CTDSP2 | |||
|---|---|---|---|---|---|---|
| rs | algorithms | rs | algorithms | rs | algorithms | |
| Lung ADC (TCGA data) | ||||||
| miR-96 | −0.26 | D, Tn | −0.22 | D, Tc | −0.29 | D, Tc |
| miR-182 | −0.22 | D, Tn | −0.21 | D, Tc, Tn | −0.18 | Tc |
| miR-183 | −0.27 | D, Tc, Tn, Rw | −0.27 | D, Tc, Tn | −0.17 | Tn |
| Squamous cell lung cancer (TCGA data) | ||||||
| miR-96 | −0.34 | D, Tn | −0.10 | D,Tc | −0.28 | D, Tc |
| miR-182 | −0.16 | D, Tn | −0.10 | D,Tc,Tn, | −0.14 | Tc |
| miR-183 | −0.27 | D, Tc, Tn, Rw | −0.24 | D,Tc,Tn | −0.14 | Tn |
| (P<0.01 for all cases) | ||||||
| C | CTDSPL | CTDSP1 | CTDSP2 |
|---|---|---|---|
| Non-small squamous cell lung cancer (qPCR) | |||
| miR-96 | −0.26 | −0.34 (P=0, 08) | −0.62 (P<0.001) |
| miR-182 | −0.03 | −0.24 | −0.42 (P<0.05) |
| miR-183 | -0.08 | −0.16 | −0.35 (P=0.065) |
| miR-26a | 0.23 | 0.16 | 0.37 (P<0.05) |
| miR-26b | 0.25 | 0.15 | 0.39 (P<0.05) |
| (P>0.05 for all cases, unless otherwise specified) | |||
(A) The comparison of the mRNA levels of CTDSPs and RB1 in primary ADC and SCC samples. (B) Results of joint analysis of TCGA and microRNA target prediction resources performed by the CrossHub tool (https://sourceforge.net/projects/crosshub/). Algorithms: D – DIANA microT, Tc – TargetScan conservative sites, Tn – TargetScan non-conservative sites, Rw – miRTarBase weak evidence. (C) The comparison of the mRNA levels of CTDSPs and microRNAs in NSCLC.