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. Author manuscript; available in PMC: 2019 Dec 14.
Published in final edited form as: Cell Rep. 2019 Nov 26;29(9):2621–2633.e4. doi: 10.1016/j.celrep.2019.10.099

Figure 2. Zc3h10 Promotes Thermogenic Gene Program In Vitro.

Figure 2.

(A) (Left) qRTqPCR and immunoblotting for Zc3h10KD in BAT cells.

(B) (Left) qRT-PCR for indicated genes in forskolin-treated differentiated Zc3h10KD BAT cells (Middle) Immunoblotting for indicated proteins. (Right) ORO staining of BAT cells (20× magnification) at day 5 of differentiation.

(C) OCR (pMol/min) measured using Seahorse XF24 analyzer in Zc3h10 KD BAT cells.

(D) (Left) Quantification of mitochondria number in Zc3h10KD BAT cells. (Middle) Mitochondria staining and quantification of MitoTraker Green signals in shZc3h10-infected cells (shown in red, n = 30).

(E) qRT-PCR/western blot for Zc3h10 in 3T3-L1 cells infected with Zc3h10 adenovirus.

(F) qRT-PCR/western blot for indicated genes in 3T3-L1 cells after 6 h of forskolin (10 μM) treatment.

(G) OCR (pMol/min) measured in Zc3h10-overexpressing 3T3-L1 cells.

(H) qRT-PCR for Zc3h10 in primary white adipocytes differentiated from SVF of WT and AQ-Zc3h10 TG.

(I) qRT-PCR/western blot for indicated genes in primary white adipocytes after 6 h of forskolin (10 μM) treatment.

(J) OCR measured by Seahorse assay.

Data presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.