Fig. 6.

Treatment of HSA^LR mice increases Mbnl1 and Mbnl2 levels. (A–H) Western blotting analyses of protein extracts from quadriceps (qd) (A, C, E, and G) or gastrocnemius (gt) (B, D, F, and H) of FVB control mice (n = 4, green bars), untreated HSA^LR model mice (n = 6, red bars), or model mice injected for 7 d with CQ at 25 mg/kg (n = 3, dark blue bars) or 50 mg/kg CQ (n = 5, pale blue bars). Protein levels are relative to Gapdh, which was used as an internal control. Representative blots used for quantification are shown below bar graphs. Because Clcn1 and Mbnl2 proteins were detected in the same blots, the internal control Gapdh was the same (duplicated blot in D and H). All comparisons are relative to HSA^LR mice treated with the vehicle PBS (DM). *P < 0.05, **P < 0.01 according to Student’s t test. CNT, control.