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. 2019 Dec 13;20:605. doi: 10.1186/s12891-019-2967-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — MCM3AP-AS1 over-expression led to up-regulated HMGB1. HMGB1 is a direct downstream target of miR-142-3p. The effects of MCM3AP-AS1 and miR-142-3p over-expression on HMGB1 expression at mRNA and protein levels were analyzed by qPCR (a) and western blot (b), respectively. Gene names on X-axis indicated the over-expression of this gene. C on X-axis, control cells, cells without transfection, NC on X-axis, negative control, cells transfected with empty vector or NC miRNA. Experiments were repeated 3 times and data were expressed as mean values. *, p < 0.05