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. 2019 Dec 10;14:9745–9761. doi: 10.2147/IJN.S230693

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© 2019 Qu et al.

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Identification of the RGD.Ad-ING4-IL-24 vector. (A) Gel electrophoresis of homologous recombinant adenovirus clones: Line 1, pAdTrack-CMV-ING4-polyA+promoter-IL-24 plasmid; Line 2, pAdEasy-1 adenoviral backbone plasmid; Line 3~8, the 6 selected clones. (B) Gel electrophoresis of genes ING4, IL-24 and poly+promoter amplified by PCR: Line 1, ING4 cDNA fragments amplified by PCR; Line 2, negative control group compared to ING4 cDNA fragments amplified by PCR; Line 3, polyA+promoter cDNA fragments amplified by PCR; Line 4, negative control group compared to polyA+promoter cDNA fragments amplified by PCR; Line 5, IL-24 cDNA fragments amplified by PCR; Line 6, negative control group compared to IL-24 cDNA fragments amplified by PCR; M, DL2000 marker. (C) The ING4 expression in QBI-293A cells analyzed by Western blotting. (D) The IL-24 secretion from QBI-293A cells detected by ELISA. Statistically significant in comparison with PBS, *p<0.05.

Abbreviations: RGD, Arg-Gly-Asp; Ad, adenovirus; ING4, inhibitor of growth 4; IL-24, interleukin-24; CMV, cytomegalovirus; PCR, polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.