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. 2019 Dec 11;7:e8269. doi: 10.7717/peerj.8269

Figure 4. Detection of pri-miRNA in the phloem.

Figure 4

Detection of pri-miRNA (A) and TCTP RNA (B) in RNA preparations from phloem exudate and leaf samples. Reverse transcription was carried out with oligo-dT as a primer; pri-miRNA sequences and TCTP RNA were amplified with pairs of specific primers. (C) Detection of pri-miR319a transport across the graft union. Reverse transcription was carried out with oligo-dT as a primer. TCTP and pri-miR319a were amplified with specific primers designed to anneal to respective sequences of C. maxima, but not C. melo. The sizes of pri-miR319a-specific products in A and C are 162 bp and 276 bp, respectively, as these products were obtained with different pairs of specific primers (Table S1). Water, a control sample without input RNA. M, DNA size marker; lengths of individual DNA fragments in bp are indicated.