Figure 4.

rTRD01 improves larval turning in a Drosophila model of ALS. (A) Larvae expressing D42 GAL4-driven human wild-type (TDP-43^WT) or G298S mutant TDP-43 (TDP-43_G298S) take significantly longer to turn over following a ventral-up inversion. D42 GAL4 crossed with w¹¹¹⁸ larvae were used as control. Asterisks indicate statistical difference assessed by Kruskal-Wallis (p < 0.0001, n = 20–30). (B) rTRD01 has no significant effect on the turning time of larvae expressing TDP-43^WT. (C) rTRD01 reduced the turning time of larvae expressing TDP-43^G298S from ∼19s to less than ∼13s, on average from n ≥ 20 larvae tested. rTRD01 was fed at a concentration of 20 μM in the food. Data is presented as mean ± SEM. Asterisks indicate statistically significant difference as assessed by a Mann-Whitney test (p < 0.0001, n ≥ 20).