Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Yeast strains | ||
| Schizosaccharomyces pombe: h− wild-type strain 972 | Chang lab collection | FC15 |
| S. pombe: h− cdc25-22 | Chang lab collection | FC342 |
| S. pombe: h− sec6-GFP-ura4+ leul-32 ura4- | Chang lab collection | FC1037 |
| S. pombe: h− ssp2::ura4+ leul-32 ura4-D18 | Chang lab collection | FC1503 |
| S. pombe: h-gad8::ura4+ leu1-32 ura4-D18 | Chang lab collection | FC1507 |
| S. pombe: h-gad8::ura4+ leu1-32 ura4-D18 | Chang lab collection | FC1507 |
| S. pombe: h− pmk1::ura4+ leu1-32 ura4-D18 | Chang lab collection | FC1551 |
| S. pombe: h− sty1::ura4+ leu1-32 ura4-D18 | Chang lab collection | FC1568 |
| S. pombe: h− cch1::ura4+ leu1-32 ura4-D18 | Chang lab collection | FC1596 |
| S. pombe: h− fim1-meGFP-kanMX6 | Chang lab collection | FC1897 |
| S. pombe: h− rga4-RFP::kanMX6 leu1-32 ura4-D18 | Chang lab collection | FC1902 |
| S. pombe: h− ost1-GFP::ura4+ rlcl-RFP::ura4+leu1-32 ura4-D18 | Chang lab collection | FC1919 |
| S. pombe: h− trn1-GFP::kanMX6 ade6- leu1-32 ura4-D18 | Chang lab collection | FC2086 |
| S. pombe: h− yop1-GFP::kanMX6 ade6- leu1-32 ura4-D18 | Chang lab collection | FC2087 |
| S. pombe: h− leu1:2xGFP-bgs4 leu1+ ura4+ | Chang lab collection | FC2255 |
| S. pombe: h− gma12-GFP-ura4+ leu1:tdTom-bgs1 leu1+ ura4+ | Chang lab collection | FC2277 |
| S. pombe: h+ pom1-tomato-natMX, cdr2-GFP-kanMX ade6- leu1-32 ura4-D18 | Chang lab collection | FC2678 |
| S. pombe: h− gpd1::kanMX leu1-32 | Chang lab collection | FC2810 |
| S. pombe: h− tdh1-dendra2:ura4 ade6- leu1-32 ura4-D18 | Chang lab collection | FC2840 |
| S. pombe: h+ hta1-mCherry:kanMX GFP-atb2:kanMX ade6- leu1-32 ura4- | Chang lab collection | FC2859 |
| S. pombe: h+ GFP-atb2:kanMX ade6- leu1-32 ura4-D18 | Chang lab collection | FC2861 |
| S. pombe: h− leu1-tomato-bgsl psy1-GFP-leu1 | Chang lab collection | FC2913 |
| S. pombe: h+ act1p::1XE2C:HygR ura4-D18 leu1-32 ade6-M210 his7-366 | Chang lab collection | FC3186 |
| S. pombe: h− rps802-GFP::kanMX leu1-32 ura4-D18 ade6-M210 | Chang lab collection | FC3208 |
| S. pombe: h− rps2-GFP::kanMX leu1-32 ura4-D18 ade6-M210 | Chang lab collection | FC3209 |
| S. pombe: h+ rpl1601-GFP::kanR leu1-32 ura4-D18 ade6-M210 | Chang lab collection | FC3210 |
| S. pombe: h− rpl2801-GFP::kanMX leu1-32 ura4-D18 ade6- | Chang lab collection | FC3212 |
| S. pombe: h+ rps2401-GFP::kanR leu1-32 ura4-D18 ade6-M216 | Chang lab collection | FC3213 |
| S. pombe: h+ rpl3001-GFP::kanR leu1-32 ura4-D18 ade6-M216 | Chang lab collection | FC3215 |
| S. pombe: h− [pREP42-pnmt1-PfV-GS-Sapphire-tnmt1] leu1-32 ura4-D18 ade6-M216 | This manuscript | FC3238 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| YE5S rich media | Sunrise Science | Cat. #YES-225 |
| Edinburgh Minimal Media (EMM) | US Biological | Cat. #E2205 |
| Difco agar | Fisher Scientific | Cat. # DF0812-07-1 |
| D-Sorbitol | Sigma-Aldrich | Cat. #S1876 |
| D-Glucose | Sigma-Aldrich | Cat. #G8270 |
| Adenine | Sigma-Aldrich | Cat. #A8626 |
| Leucine | Sigma-Aldrich | Cat. #L8000 |
| Histidine | Sigma-Aldrich | Cat. #H8000 |
| Cycloheximide | Sigma-Aldrich | Cat. #C7698 |
| Brefeldin A | Sigma-Aldrich | Cat. #B7651 |
| Wheat Germ Agglutinin, Alexa Fluor 488 Conjugate | Life Technologies | Cat. # W11261 |
| Fluorescein isothiocyanate (FITC) | Sigma-Aldrich | Cat. # F7250 |
| Propidium iodide (PI) | Sigma-Aldrich | Cat. #P4170 |
| RNase A | ThermoFisher | Cat. #EN0531 |
| Bovine serum albumin | Sigma-Aldrich | Cat. #A3608 |
| Software and Algorithms | ||
| Morphometries v. 1.1 | (Tyanova et al., 2016) | https://simtk.org/projects/morphometrics |
| DeepCell | (Van Valen et al. 2017) | https://hub.docker.com/r/vanvalen/deepcell/ |
| ImageJ v. 1.51 | NIH | https://imagej.nih.gov |
| MOSAIC for ImageJ | (Shivanandan et al., 2013) | https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-14-349 |
| μManager v. 1.4 | (Edelstein et al., 2014a) | N/A |
| MATLAB 2017a | MathWorks | www.mathworks.com/products/matlab.html |
| Other | ||
| 0.45-μm filter | Millipore | HAWP04700 |
| Microfluidic perfusion plates | CellASIC | Cat. #Y04C |
| ONIX microfluidic platform | CellASIC | N/A |
| Nikon Eclipse Ti-E inverted fluorescence microscope | Nikon | N/A |
| Yokogawa confocal spinning disk CSU-10 | Yokogawa | N/A |
| Andor Diskovery multi-modal system | Andor | N/A |
| Borealis illumination system | Andor | N/A |
| Integrated laser system (488 nm, 561 nm) | Andor | N/A |
| OBIS 488 nm laser | Coherent | N/A |
| EM-CCD camera | Hamamatsu | N/A |
| iXon Ultra EM-CCD camera | Andor | N/A |
| Zyla 4.2 sCMOS camera | Andor | N/A |
| Active-control environmental chamber | Haison Technology | N/A |
| Active-control environmental chamber | Okolab | N/A |