Yeast strains |
Schizosaccharomyces pombe: h− wild-type strain 972 |
Chang lab collection |
FC15 |
S. pombe: h− cdc25-22
|
Chang lab collection |
FC342 |
S. pombe: h− sec6-GFP-ura4+ leul-32 ura4-
|
Chang lab collection |
FC1037 |
S. pombe: h− ssp2::ura4+ leul-32 ura4-D18
|
Chang lab collection |
FC1503 |
S. pombe: h-gad8::ura4+ leu1-32 ura4-D18
|
Chang lab collection |
FC1507 |
S. pombe: h-gad8::ura4+ leu1-32 ura4-D18
|
Chang lab collection |
FC1507 |
S. pombe: h− pmk1::ura4+ leu1-32 ura4-D18
|
Chang lab collection |
FC1551 |
S. pombe: h− sty1::ura4+ leu1-32 ura4-D18
|
Chang lab collection |
FC1568 |
S. pombe: h− cch1::ura4+ leu1-32 ura4-D18
|
Chang lab collection |
FC1596 |
S. pombe: h− fim1-meGFP-kanMX6
|
Chang lab collection |
FC1897 |
S. pombe: h− rga4-RFP::kanMX6 leu1-32 ura4-D18
|
Chang lab collection |
FC1902 |
S. pombe: h− ost1-GFP::ura4+ rlcl-RFP::ura4+leu1-32 ura4-D18
|
Chang lab collection |
FC1919 |
S. pombe: h− trn1-GFP::kanMX6 ade6- leu1-32 ura4-D18
|
Chang lab collection |
FC2086 |
S. pombe: h− yop1-GFP::kanMX6 ade6- leu1-32 ura4-D18
|
Chang lab collection |
FC2087 |
S. pombe: h− leu1:2xGFP-bgs4 leu1+ ura4+
|
Chang lab collection |
FC2255 |
S. pombe: h− gma12-GFP-ura4+ leu1:tdTom-bgs1 leu1+ ura4+
|
Chang lab collection |
FC2277 |
S. pombe: h+ pom1-tomato-natMX, cdr2-GFP-kanMX ade6- leu1-32 ura4-D18
|
Chang lab collection |
FC2678 |
S. pombe: h− gpd1::kanMX leu1-32
|
Chang lab collection |
FC2810 |
S. pombe: h− tdh1-dendra2:ura4 ade6- leu1-32 ura4-D18
|
Chang lab collection |
FC2840 |
S. pombe: h+ hta1-mCherry:kanMX GFP-atb2:kanMX ade6- leu1-32 ura4-
|
Chang lab collection |
FC2859 |
S. pombe: h+ GFP-atb2:kanMX ade6- leu1-32 ura4-D18
|
Chang lab collection |
FC2861 |
S. pombe: h− leu1-tomato-bgsl psy1-GFP-leu1
|
Chang lab collection |
FC2913 |
S. pombe: h+ act1p::1XE2C:HygR ura4-D18 leu1-32 ade6-M210 his7-366
|
Chang lab collection |
FC3186 |
S. pombe: h− rps802-GFP::kanMX leu1-32 ura4-D18 ade6-M210
|
Chang lab collection |
FC3208 |
S. pombe: h− rps2-GFP::kanMX leu1-32 ura4-D18 ade6-M210
|
Chang lab collection |
FC3209 |
S. pombe: h+ rpl1601-GFP::kanR leu1-32 ura4-D18 ade6-M210
|
Chang lab collection |
FC3210 |
S. pombe: h− rpl2801-GFP::kanMX leu1-32 ura4-D18 ade6-
|
Chang lab collection |
FC3212 |
S. pombe: h+ rps2401-GFP::kanR leu1-32 ura4-D18 ade6-M216
|
Chang lab collection |
FC3213 |
S. pombe: h+ rpl3001-GFP::kanR leu1-32 ura4-D18 ade6-M216
|
Chang lab collection |
FC3215 |
S. pombe: h− [pREP42-pnmt1-PfV-GS-Sapphire-tnmt1] leu1-32 ura4-D18 ade6-M216
|
This manuscript |
FC3238 |
Chemicals, Peptides, and Recombinant Proteins |
YE5S rich media |
Sunrise Science |
Cat. #YES-225 |
Edinburgh Minimal Media (EMM) |
US Biological |
Cat. #E2205 |
Difco agar |
Fisher Scientific |
Cat. # DF0812-07-1 |
D-Sorbitol |
Sigma-Aldrich |
Cat. #S1876 |
D-Glucose |
Sigma-Aldrich |
Cat. #G8270 |
Adenine |
Sigma-Aldrich |
Cat. #A8626 |
Leucine |
Sigma-Aldrich |
Cat. #L8000 |
Histidine |
Sigma-Aldrich |
Cat. #H8000 |
Cycloheximide |
Sigma-Aldrich |
Cat. #C7698 |
Brefeldin A |
Sigma-Aldrich |
Cat. #B7651 |
Wheat Germ Agglutinin, Alexa Fluor 488 Conjugate |
Life Technologies |
Cat. # W11261
|
Fluorescein isothiocyanate (FITC) |
Sigma-Aldrich |
Cat. # F7250 |
Propidium iodide (PI) |
Sigma-Aldrich |
Cat. #P4170 |
RNase A |
ThermoFisher |
Cat. #EN0531 |
Bovine serum albumin |
Sigma-Aldrich |
Cat. #A3608 |
Software and Algorithms |
Morphometries v. 1.1 |
(Tyanova et al., 2016) |
https://simtk.org/projects/morphometrics |
DeepCell |
(Van Valen et al. 2017) |
https://hub.docker.com/r/vanvalen/deepcell/ |
ImageJ v. 1.51 |
NIH |
https://imagej.nih.gov |
MOSAIC for ImageJ |
(Shivanandan et al., 2013) |
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-14-349 |
μManager v. 1.4 |
(Edelstein et al., 2014a) |
N/A |
MATLAB 2017a |
MathWorks |
www.mathworks.com/products/matlab.html |
Other |
0.45-μm filter |
Millipore |
HAWP04700 |
Microfluidic perfusion plates |
CellASIC |
Cat. #Y04C |
ONIX microfluidic platform |
CellASIC |
N/A |
Nikon Eclipse Ti-E inverted fluorescence microscope |
Nikon |
N/A |
Yokogawa confocal spinning disk CSU-10 |
Yokogawa |
N/A |
Andor Diskovery multi-modal system |
Andor |
N/A |
Borealis illumination system |
Andor |
N/A |
Integrated laser system (488 nm, 561 nm) |
Andor |
N/A |
OBIS 488 nm laser |
Coherent |
N/A |
EM-CCD camera |
Hamamatsu |
N/A |
iXon Ultra EM-CCD camera |
Andor |
N/A |
Zyla 4.2 sCMOS camera |
Andor |
N/A |
Active-control environmental chamber |
Haison Technology |
N/A |
Active-control environmental chamber |
Okolab |
N/A |