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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Eur Neuropsychopharmacol. 2019 Oct 1;29(11):1288–1294. doi: 10.1016/j.euroneuro.2019.09.004

Figure 1.

Figure 1.

GLAST or GLT-1 knockdown in mouse infralimbic cortex (IL) altered electrophysiological properties of layer V pyramidal neurons. (a) Bar graphs showing a significant reduction of the resting membrane potential (RMP) in siRNA-treated mice compared to controls. (b) I/V curves showed no changes in neuronal membrane conductance between groups. (c) Representative examples of action potential firing evoked by a depolarizing pulse (0.1–10 mA, 2 ms) recorded in current-clamp conditions in each group. (d) Bar graph showing the firing rate after applying increasing depolarizing current pulses to layer V neurons, indicating a higher excitability in siRNA-treated mice. (e) Representative traces of evoked EPSCs in aCSF, GLAST and GLT-1 siRNA-treated mice, showing significant changes in EPSC waveform. Note that the stimulation artifact has been removed from the traces. (f) 10–90% rise and decay times of evoked EPSCs showing a significant slowing-down in GLAST and GLT-1 knockdown mice. Data are presented as mean±SEM. P<0.05 (*), P<0.01 (**), and P<0.001 (***) vs controls, post-hoc Tukey’s test after one-way ANOVA.