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. Author manuscript; available in PMC: 2020 Apr 4.
Published in final edited form as: Sci Immunol. 2019 Oct 4;4(40):eaay7501. doi: 10.1126/sciimmunol.aay7501

Figure 6. REV-ERBα deficiency differentially affects cytokine production in ILC3 subsets.

Figure 6.

A) IL-17A and IL-22 production in ILC3 subsets in response to in vitro stimulation with IL1β and IL-23 (10 ng/ml). Left: Representative intracellular staining of IL-22 and IL-17A. Right: Frequency and expression levels (gMFI) of IL-22+ and IL-17A+ ILC3s (right). B) IFN-g production in NKp46+ ILC3s in response to in vitro stimulation with a mixture of IL1β, IL-23, IL-12, and IL-18. C) IL-17 and IL-22 secretion by ILC3 subsets on day 2 of CDI. D) qPCR analysis of relative bacterial load translocated to mLNs on day 2 of CDI. rDNA, ribosomal DNA. Statistical analysis was performed using one-way ANOVA with multiple comparisons test (A and B) or Student’s t-test (C and D). Bars indicate means (±SD). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data are representative of two independent experiments (n = 3 to 5 per group per experiment) (A to C) or pooled from two independent experiments (n = 4 or 5 per group per experiment) (D).