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. Author manuscript; available in PMC: 2020 Jun 15.
Published in final edited form as: Cancer Res. 2019 Oct 29;79(24):6126–6138. doi: 10.1158/0008-5472.CAN-19-0950

Figure 1.

Figure 1.

Knockout of Mcl-1 suppresses cancer cell growth via downregulation of Akt activity. A-E, Mcl-1 was knocked out from H1299 cells using CRISPR/Cas9, followed by analysis of Mcl-1 expression by Western blot (A), growth curve (B), colony formation (C), human phospho-kinase array (1: Akt S473; 2: Akt T308) (D), and quantification of human phospho-kinase by image J (E). Data represent the mean ± SD. *P < 0.05, ***P < 0.001, by 2-tailed t test. (F) Mcl-1 was knocked out from H1299 cells, or knocked down from H460 cells, followed by Western blotting analysis of Mcl-1, pAkt, pmTOR and p-p70S6K.